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Periodic boundary conditions were used with a cutoff of 10 for non-bonded interactions and the Particle Mesh Ewald method for long-range electrostatics

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Based on a comprehensive analysis of the literature, we propose that BACH1 is a typical context-dependent transcription factor, and its function is strongly regulated by the intracellular redox state: ROS levels and heme metabolism significantly influence the expression and activity of BACH1